Department of Medical Biochemistry, Faculty of Medicine in Hradec Králové, Charles University in Prague, Hradec Králové, Czech Republic.
Physiol Res. 2011;60(4):667-77. doi: 10.33549/physiolres.932083. Epub 2011 May 16.
Cellular response to ionizing radiation-induced damage depends on the cell type and the ability to repair DNA damage. Some types of cells undergo apoptosis, whereas others induce a permanent cell cycle arrest and do not proliferate. Our study demonstrates two types of response of embryonic diploid fibroblasts WI-38 to ionizing radiation. In the WI-38 cells p53 is activated, protein p21 increases, but the cells are arrested in G2 phase of cell cycle. Some of the cells die by apoptosis, but in remaining viable cells p16 increases, senescence associated DNA-damage foci occur, and senescence-associated beta-galactosidase activity increases, which indicate stress-induced premature senescence.
细胞对电离辐射诱导损伤的反应取决于细胞类型和修复 DNA 损伤的能力。一些类型的细胞发生凋亡，而其他细胞则诱导永久的细胞周期阻滞，不再增殖。我们的研究表明，胚胎二倍体成纤维细胞 WI-38 对电离辐射有两种反应类型。在 WI-38 细胞中，p53 被激活，蛋白 p21 增加，但细胞被阻滞在细胞周期的 G2 期。一些细胞通过凋亡死亡，但在剩余的存活细胞中，p16 增加，出现衰老相关的 DNA 损伤焦点，衰老相关的β-半乳糖苷酶活性增加，表明应激诱导的过早衰老。
