[半乳糖凝集素-9和Tim-3在哮喘小鼠肺组织中的表达]

[Expression of Galectin-9 and Tim-3 in lungs of mice with asthma].

作者信息

Zhang Zhi-Ying, Luan Bin, Feng Xiao-Xia

机构信息

Department of Pediatrics, Third Hospital Affiliated to Zhengzhou University, Zhengzhou 450052, China.

出版信息

Zhongguo Dang Dai Er Ke Za Zhi. 2011 May;13(5):406-10.

PMID:21575348

Abstract

OBJECTIVE

To study the expression of Galectin-9 and Tim-3 in lungs of mice with asthma and the effect of rosiglitazone (PPAR-γ agonist) on their expression.

METHODS

Fortyfive BALB/c SPF female mice were randomized into control group and asthma groups with and without rosiglitazone intervention. After ovalbumin stimulation and rosiglitazone intervention the pathological changes of the lung tissues were observed. Galectin-9 and Tim-3 mRNA levels in lung tissues were determined using RT-PCR. The levels of IL-4 and IFN-γ in peripheral blood were measured using ELISA.

RESULTS

The expression of Galectin-9 and Tim-3 mRNA of lung tissues in the untreated asthma group increased significantly compared with the control and the rosiglitazone treated groups (P<0.05). A significantly increased blood expression of IL-4 and a significantly decreased blood expression of IFN-γ were found in the untreated asthma group compared with the control and the rosiglitazone-treated groups (P<0.05). The expression of Galectin-9 and Tim-3 mRNA was positively correlated with blood IL-4 level (r=0.792, r=0.794 respectively; P<0.05), but negatively correlated with blood IFN-γ level (r=-0.692, r=-0.757 respectively; P<0.05).

CONCLUSIONS

Galectin-9 and Tim-3 mRNA levels in lungs increase in mice with asthma and significantly correlate with the levels of blood Th1/Th2 cytokines. This suggests that Galectin-9 and Tim-3 are closely related to inflammatory process in asthma. Rosiglitazone treatment may decrease the expression of Galectin-9 and Tim-3.

摘要

目的

研究半乳糖凝集素-9（Galectin-9）和T细胞免疫球蛋白黏蛋白分子-3（Tim-3）在哮喘小鼠肺组织中的表达及罗格列酮（PPAR-γ激动剂）对其表达的影响。

方法

将45只BALB/c SPF级雌性小鼠随机分为对照组、哮喘组和罗格列酮干预哮喘组。经卵清蛋白激发及罗格列酮干预后，观察肺组织病理变化，采用逆转录聚合酶链反应（RT-PCR）检测肺组织中Galectin-9和Tim-3 mRNA水平，采用酶联免疫吸附测定（ELISA）检测外周血白细胞介素-4（IL-4）和干扰素-γ（IFN-γ）水平。

结果

未治疗哮喘组肺组织Galectin-9和Tim-3 mRNA表达较对照组及罗格列酮治疗组显著升高（P<0.05）。未治疗哮喘组外周血IL-4水平较对照组及罗格列酮治疗组显著升高，IFN-γ水平显著降低（P<0.05）。Galectin-9和Tim-3 mRNA表达与外周血IL-4水平呈正相关（r分别为0.792、0.794；P<0.05），与外周血IFN-γ水平呈负相关（r分别为-0.692、-0.757；P<0.05）。