Su Xiao-juan, Tang Zhi-feng, Li Qiang, Li Hua-long, Li Ling-ling, Liu Min, Zhou Yong-ning
Department of Gastroenterology, First Hospital of Lanzhou University, Lanzhou 730000, China.
Zhonghua Zhong Liu Za Zhi. 2011 Apr;33(4):276-9.
To investigate the expression of RhoA and NF-κB in gastric carcinoma and their correlation with clinicopathological fearures. To determine the effective prognostic factors of long-term suivival of gastric carcinoma patients.
The role of RhoA and NF-κB in gastric carcinoma was assessed by tissue array technology and the levels of RhoA and NF-κB expression in paraffin-embedded tissues was quantified by immunohistochemistry from 189 cases of gastric carcinoma, 54 cases of their adjacent tissues, and 32 cases of normal gastric mucosa. The prognosis of gastric carcinoma was evaluated by Kaplan-Meier survival analysis and Cox multivariate regression analysis.
The positive rates of RhoA expression were 84.7%, 68.5% and 65.6% in gastric carcinoma, adjacent tissues and normal mucosa, respectively. The expression of RhoA in gasric carcinoma was significantly higher than that in adjacent tissues and normal mucosa (P < 0.05). The positive rates of NF-κB expression were 75.1%, 42.6% and 15.6%% in gastric carcinoma, adjacent tissues and normal mucosa, respectively. The expression of NF-κB in gasric carcinoma was significantly higher than that in adjacent tissues and normal mucosa (P < 0.05). RhoA was positively linked with NF-κB (r = 0.203, P = 0.005). In gastric carcinoma, the expression of RhoA was related with depth of invasion (P < 0.05), and the expression of NF-κB was related with depth of invasion and lymph node metastasis (P < 0.05). The Kaplan-Meier survival analysis showed that the tumor size, lymph node metastasis, depth of invasion, expression of RhoA and NF-κB can shorten the cumulative survival rate. With these paramaters entering the Cox multivariate regression analysis mode, it was revealed that expression of NF-κB, lymph node metastasis and depth of invasion are independent prognostic factors.
The overexpression of RhoA and NF-κB is involved in the occurrence and development of gastric carcinoma. RhoA is positively linked with NF-κB. They are correlated with the invasion and metastasis of gastric carcinoma. The expression of NF-κB, lymph node metastasis, depth of invasion are independent prognostic factors playing an important role in prediction of the clinical outcome after radical resection of gastric carcinoma.
探讨RhoA和NF-κB在胃癌中的表达及其与临床病理特征的相关性。确定胃癌患者长期生存的有效预后因素。
采用组织芯片技术评估RhoA和NF-κB在胃癌中的作用，通过免疫组化对189例胃癌、54例癌旁组织及32例正常胃黏膜石蜡包埋组织中RhoA和NF-κB的表达水平进行定量分析。采用Kaplan-Meier生存分析和Cox多因素回归分析评估胃癌的预后。
RhoA在胃癌、癌旁组织及正常黏膜中的阳性表达率分别为84.7%、68.5%和65.6%。胃癌中RhoA的表达显著高于癌旁组织和正常黏膜（P<0.05）。NF-κB在胃癌、癌旁组织及正常黏膜中的阳性表达率分别为75.1%、42.6%和15.6%。胃癌中NF-κB的表达显著高于癌旁组织和正常黏膜（P<0.05）。RhoA与NF-κB呈正相关（r=0.203，P=0.005）。在胃癌中，RhoA的表达与浸润深度有关（P<0.05），NF-κB的表达与浸润深度及淋巴结转移有关（P<0.05）。Kaplan-Meier生存分析显示，肿瘤大小、淋巴结转移、浸润深度、RhoA和NF-κB的表达均可缩短累积生存率。将这些参数纳入Cox多因素回归分析模型，结果显示NF-κB的表达、淋巴结转移及浸润深度是独立的预后因素。
RhoA和NF-κB的过表达参与了胃癌的发生发展。RhoA与NF-κB呈正相关。它们与胃癌的侵袭和转移相关。NF-κB的表达、淋巴结转移、浸润深度是独立的预后因素，在预测胃癌根治术后临床结局中起重要作用。
