Department of Biochemistry, Institute of Chemistry, Federal University of Rio de Janeiro, Cidade Universitária, Centro de Tecnologia, Bl. A, Sl. 549, Ilha do Fundão, 21949-900, Rio de Janeiro, Brazil.
Bioresour Technol. 2011 Jul;102(14):7043-50. doi: 10.1016/j.biortech.2011.04.037. Epub 2011 Apr 27.
The hydrolysis of effluent from a poultry slaughterhouse containing 800 mg oil and grease (O&G)/L was conducted with 1% (w/v) of an enzymatic pool obtained by solid-state fermentation with the fungus Penicillium restrictum. The chromatographic evaluation of the lipid profile during hydrolysis indicated a higher concentration of acids after 4h of reaction (2954 mg/L), with a predominance of oleic, palmitic, and linoleic acids. Effluent aliquots were collected after 4, 8, and 24h of hydrolysis and tested for anaerobic biodegradation in sequential batches. An adaptation of the biomass was observed, both in the control experiment (with non-hydrolyzed raw effluent) and in the experiments with enzymatically pre-treated effluent. The specific methane production in the control experiment was 0.248 L CH(4)/g COD(consumed), and in the experiment with effluent pre-treated for 4h, this production was 0.393 L CH(4)/g COD(consumed), indicating a higher methane production after enzymatic hydrolysis.
采用固态发酵真菌藤黄青霉得到的酶制剂(1%,w/v),对含有 800mg 油和脂肪(O&G)/L 的家禽屠宰废水进行水解。水解过程中对脂类成分的色谱评估表明,反应 4 小时后酸的浓度更高(2954mg/L),其中油酸、棕榈酸和亚油酸占优势。水解 4、8 和 24 小时后收集废水样本,在连续批次中进行厌氧生物降解测试。在控制实验(未水解的原废水)和酶预处理废水中都观察到生物量的适应性。在控制实验中,甲烷的比产率为 0.248L CH4/g COD(消耗),在预处理 4 小时的废水中,甲烷的比产率为 0.393L CH4/g COD(消耗),表明酶解后甲烷的产量更高。