Hemalatha N, Rajesh M K, Narayanan N K
Department of Information Technology, School of Information Science and Technology, Kannur University Campus, Kannur 670002, Kerala, India.
Int J Bioinform Res Appl. 2011;7(2):162-7. doi: 10.1504/IJBRA.2011.040094.
In this study, we carried out genome-wide analyses to explore expansin gene family in the genome of indica rice. Reference nucleotides were chosen as query sequences for searches in the indica rice genome database. Clones having genomic sequences similar to expansin were taken and converted to amino acid sequences. Putative sequences were subjected to PROSITE and Pfam databases, and 21 signature-sequences-related expansin gene family was obtained. The presence of transmembrane domains was also predicted for all 21 expansin proteins. A phylogenetic tree was generated from the alignments of the proteins sequences to examine the phylogenetic relationship of indica rice expansin proteins.
在本研究中，我们进行了全基因组分析，以探索籼稻基因组中的扩展蛋白基因家族。选择参考核苷酸作为查询序列，在籼稻基因组数据库中进行搜索。获取具有与扩展蛋白相似基因组序列的克隆，并将其转化为氨基酸序列。将推定序列提交至PROSITE和Pfam数据库，获得了21个与特征序列相关的扩展蛋白基因家族。还对所有21种扩展蛋白进行了跨膜结构域预测。根据蛋白质序列比对生成系统发育树，以研究籼稻扩展蛋白的系统发育关系。
