Przylipiak A, Kiesel L, Habenicht A J, Przylipiak M, Runnebaum B
Department of Gynecology and Obstetrics, University of Heidelberg, F.R.G.
Mol Cell Endocrinol. 1990 Feb 12;69(1):33-9. doi: 10.1016/0303-7207(90)90086-n.
The stimulatory effect of exogenously administered potato 5-lipoxygenase (0.1-0.3 U/2 ml) on luteinizing hormone (LH) release was demonstrated in rat anterior pituitary cells in a superfusion system. Nordihydroguaiaretic acid (NDGA), an inhibitor of 5-lipoxygenase, abolished the effect of the enzyme on LH secretion. The secretory effect on LH after 5-lipoxygenase administration was biphasic and dependent on Ca2+ indicating that 5-lipoxygenase affects LH release through its oxygenation reaction. Another series of experiments demonstrated that activation of 5-lipoxygenase, expressed as production of leukotriene (LT) B4 and C4 (728 +/- 127 pg/10(6) cells and 178 +/- 23 pg/10(6) cells, respectively) occurs in rat pituitary cells after addition of Ca2+ ionophore A23187. However, LTB4 and LTC4 were not formed by pituitary cells that had previously been desensitized by gonadotropin-releasing hormone (GnRH), the physiological ligand of LH release. These results are consistent with a role of 5-lipoxygenase metabolites in the mechanism of GnRH-induced LH secretion.
在外源给予马铃薯5-脂氧合酶（0.1 - 0.3 U/2 ml）对大鼠垂体前叶细胞促黄体生成素（LH）释放的刺激作用，在一个灌流系统中得到证实。5-脂氧合酶抑制剂去甲二氢愈创木酸（NDGA）消除了该酶对LH分泌的作用。给予5-脂氧合酶后对LH的分泌作用呈双相性且依赖于Ca2+，表明5-脂氧合酶通过其氧化反应影响LH释放。另一系列实验表明，在添加Ca2+离子载体A23187后，大鼠垂体细胞中5-脂氧合酶被激活，表现为白三烯（LT）B4和C4的产生（分别为728±127 pg/10(6)细胞和178±23 pg/10(6)细胞）。然而，先前已被促性腺激素释放激素（GnRH，LH释放的生理配体）脱敏的垂体细胞不会形成LTB4和LTC4。这些结果与5-脂氧合酶代谢产物在GnRH诱导的LH分泌机制中的作用一致。
