Conte D, Falaschi P, Proietti A, D'Urso R, Citarella F, Nordio M, Romanelli F, Maggi R, Motta M, Isidori A
Neuroendocrinology. 1986;43(3):428-34. doi: 10.1159/000124559.
The aim of the present study was to evaluate whether arachidonic acid metabolism may play a role on luteinizing hormone (LH) and prolactin (PRL) release directly at the pituitary level. To this purpose, exogenous arachidonic acid, alone or in presence of inhibitors of cyclooxygenase (indomethacin:IND) and lipoxygenase pathways (nordihydroguaiaretic acid:NDGA), was added to perfused rat anterior pituitary cells. PGE, PGF alpha, LH and PRL levels present in the eluate were assayed with specific RIA methods. Both PGE and PGF alpha show a dose-related response after the addition of increasing doses of arachidonic acid. The addition of 0.05 mM arachidonic acid induces an increase of LH and PRL. The addition of IND to the perfusion medium highly potentiates the stimulatory effects induced by arachidonic acid on LH and PRL release. On the contrary, the addition to the medium of either NDGA or IND plus NDGA completely reverses the stimulatory action induced by arachidonic acid alone. The present results suggest that: adenohypophyseal cells are able to metabolize exogenous arachidonic acid; arachidonic acid induces an elevation in LH and PRL levels; lipoxygenase pathway metabolite(s) are likely involved in these activities, and the site of action of arachidonic acid is at the pituitary level.
本研究的目的是评估花生四烯酸代谢是否可能直接在垂体水平对促黄体生成素（LH）和催乳素（PRL）的释放发挥作用。为此，将外源性花生四烯酸单独或与环氧化酶抑制剂（吲哚美辛：IND）和脂氧合酶途径抑制剂（去甲二氢愈创木酸：NDGA）一起添加到灌注的大鼠垂体前叶细胞中。用特定的放射免疫分析方法测定洗脱液中存在的PGE、PGFα、LH和PRL水平。添加递增剂量的花生四烯酸后，PGE和PGFα均呈现剂量相关反应。添加0.05 mM花生四烯酸可诱导LH和PRL增加。向灌注培养基中添加IND可高度增强花生四烯酸对LH和PRL释放的刺激作用。相反，向培养基中添加NDGA或IND加NDGA可完全逆转单独花生四烯酸诱导的刺激作用。目前的结果表明：腺垂体细胞能够代谢外源性花生四烯酸；花生四烯酸可诱导LH和PRL水平升高；脂氧合酶途径的代谢产物可能参与这些活动，且花生四烯酸的作用位点在垂体水平。
