Department of Biochemistry, Faculty of Medicine, Istanbul Bilim University, Istanbul, Turkey.
In Vivo. 2011 May-Jun;25(3):387-91.
Although superficial varices of lower extremities with high morbidity are common, their etiology has not been elucidated yet. Previously, it was thought that venous hypertension was responsible for such cases by causing valvular insufficiency, but recent findings indicate that the changes in the venous wall structure might be main initiating factors. Matrix metalloproteinase enzyme-2 (MMP2) is one of the enzymes known to have functions in remodelling of the extracellular matrix mainly in vascular structures.
We studied two functional gene polymorphisms in -735 and -1306 regions of matrix metalloproteinase enzyme-2 (MMP2) gene, and their effects on mRNA expression of MMP2. We used a previously defined (PCR-RFLP) method for polymorphism analyses.
CC genotype and C allele for MMP2 -735 gene region were more common in the control group and there was no significant difference between groups for MMP2 - 1306 gene polymorphisms. MMP2 mRNA levels were higher in the group that had both varices and coronary artery disease (CAD).
There was no significant effect of MMP2 polymorphisms on mRNA expression. As MMP2 mRNA levels were higher in varices patients with CAD compared to the CAD only and varices only groups, it is necessary to make advanced researches to elucidate the relationship between CAD and varices.
尽管下肢浅表静脉曲张发病率较高，但其病因尚未阐明。以前认为静脉高压通过引起瓣膜功能不全导致此类病例，但最近的发现表明静脉壁结构的变化可能是主要的起始因素。基质金属蛋白酶酶 2（MMP2）是已知在主要在血管结构中重塑细胞外基质方面具有功能的酶之一。
我们研究了基质金属蛋白酶酶 2（MMP2）基因的-735 和-1306 区域中的两个功能基因多态性及其对 MMP2 mRNA 表达的影响。我们使用了先前定义的（PCR-RFLP）方法进行多态性分析。
MMP2-735 基因区域的 CC 基因型和 C 等位基因在对照组中更为常见，MMP2-1306 基因多态性在两组之间无显著差异。同时患有静脉曲张和冠心病（CAD）的患者的 MMP2 mRNA 水平较高。
MMP2 多态性对 mRNA 表达没有显著影响。由于 CAD 合并静脉曲张患者的 MMP2 mRNA 水平高于仅 CAD 组和仅静脉曲张组，因此有必要进行更深入的研究以阐明 CAD 和静脉曲张之间的关系。
