Lupidi G, Eufemi M, Luciani S, Ferraro A, Riva F
Dipartimento di Biologia Cellulare, Università di Camerino.
Ital J Biochem. 1990 Jan-Feb;39(1):30-7.
cAMP phosphodiesterase was partially purified from pig liver nuclei and some of its properties were investigated. The enzyme is a low km PDE. Inhibition by xanthine derivatives is in line with the behaviour of PDE from other mammalian sources, while small differences are observed towards nucleotides inhibitors. The enzyme is a chromatin-bound protein although no specific location related to transcriptional function could be demonstrated.
从猪肝细胞核中部分纯化了环磷酸腺苷磷酸二酯酶，并对其一些特性进行了研究。该酶是一种低米氏常数的磷酸二酯酶。黄嘌呤衍生物对其的抑制作用与其他哺乳动物来源的磷酸二酯酶的行为一致，而在核苷酸抑制剂方面观察到一些细微差异。该酶是一种与染色质结合的蛋白质，尽管未发现与转录功能相关的特定定位。
