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牙釉质结构通过在成釉细胞中表达显性负性 RhoA 而改变。

Dental enamel structure is altered by expression of dominant negative RhoA in ameloblasts.

机构信息

Department of Anatomy and Cell Biology, University of Pennsylvania School of Dental Medicine, Philadelphia, Pa., USA.

出版信息

Cells Tissues Organs. 2011;194(2-4):227-31. doi: 10.1159/000324559. Epub 2011 May 13.

DOI:10.1159/000324559
PMID:21576911
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC3178083/
Abstract

Using in vitrotooth germ cultures and analysis by confocal microscopy, ameloblasts treated with sodium fluoride were found to have elevated amounts of filamentous actin. Because this response is reduced by inhibitors of the Rho/ROCK signaling pathway, we generated mice that express dominant negative RhoA (RhoA(DN)) in ameloblasts for in vivo analysis. Expression of the EGFP-RhoA(DN) fusion protein was evaluated by RT-PCR and immunohistochemistry, and teeth were analyzed by scanning electron microscopy. The 3 strains expressed at either low (TgEGFP-RhoA(DN)-8), intermediate (TgEGFP-RhoA(DN)-2), or high (TgEGFP-RhoA(DN)-13) levels, and the molar teeth from the 3 strains had enamel hypoplasia and surface defects. We conclude that RhoA(DN) expressed in ameloblasts interferes with normal enamel development through the pathway that is induced by sodium fluoride.

摘要

通过体外牙胚培养和共聚焦显微镜分析，发现氟化物处理的成釉细胞中丝状肌动蛋白含量增加。因为这种反应被 Rho/ROCK 信号通路抑制剂所减少，我们生成了在成釉细胞中表达显性失活 RhoA（RhoA(DN)）的小鼠用于体内分析。通过 RT-PCR 和免疫组织化学评估 EGFP-RhoA(DN)融合蛋白的表达，并通过扫描电子显微镜分析牙齿。这 3 个品系的表达水平分别为低（TgEGFP-RhoA(DN)-8）、中（TgEGFP-RhoA(DN)-2）或高（TgEGFP-RhoA(DN)-13），并且来自这 3 个品系的磨牙都有釉质发育不全和表面缺陷。我们得出结论，成釉细胞中表达的 RhoA(DN)通过氟化物诱导的途径干扰正常的釉质发育。

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本文引用的文献

1
GTPases RhoA and Rac1 are important for amelogenin and DSPP expression during differentiation of ameloblasts and odontoblasts.GTPases RhoA 和 Rac1 在成釉细胞和成牙本质细胞分化过程中对于釉原蛋白和牙本质涎磷蛋白的表达非常重要。
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Synergistic roles of amelogenin and ameloblastin.釉原蛋白和成釉蛋白的协同作用。
J Dent Res. 2009 Apr;88(4):318-22. doi: 10.1177/0022034509334749.
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Transgenic mice that express normal and mutated amelogenins.表达正常和突变釉原蛋白的转基因小鼠。
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Effects of sodium fluoride on the actin cytoskeleton of murine ameloblasts.氟化钠对小鼠成釉细胞肌动蛋白细胞骨架的影响。
Arch Oral Biol. 2005 Aug;50(8):681-8. doi: 10.1016/j.archoralbio.2004.11.021. Epub 2005 Feb 1.
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The effect of fluoride on the developing tooth.氟化物对发育中牙齿的影响。
Caries Res. 2004 May-Jun;38(3):268-76. doi: 10.1159/000077766.
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The small bovine amelogenin LRAP fails to rescue the amelogenin null phenotype.小型牛牙釉蛋白LRAP无法挽救牙釉蛋白缺失表型。
Calcif Tissue Int. 2003 Nov;73(5):487-95. doi: 10.1007/s00223-002-0036-7. Epub 2003 Sep 10.
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Specificity and mechanism of action of some commonly used protein kinase inhibitors.一些常用蛋白激酶抑制剂的特异性及作用机制
Biochem J. 2000 Oct 1;351(Pt 1):95-105. doi: 10.1042/0264-6021:3510095.
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Inhibition of RhoGAP activity is sufficient for the induction of Rho-mediated actin reorganization.抑制RhoGAP活性足以诱导Rho介导的肌动蛋白重组。
Eur J Cell Biol. 1999 Aug;78(8):539-48. doi: 10.1016/S0171-9335(99)80019-3.
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