Department of Biology, Tsurumi University School of Dental Medicine, Tsurumi, Tsurumi-ku, Yokohama 230-8501, Japan.
J Histochem Cytochem. 2012 May;60(5):376-85. doi: 10.1369/0022155412438887. Epub 2012 Feb 29.
The localization of the planar cell polarity proteins Vang12, frizzled-3, Vang11, and Celsr1 in the rat incisors was examined using immunocytochemistry. The results showed that Vang12 was localized at two regions of the Tomes' processes of inner enamel-secretory ameloblasts in rat incisors: a proximal and a distal region. In contrast, frizzled-3 was localized at adherens junctions of the proximal and distal areas of inner enamel- and outer enamel-secretory ameloblasts, where N-cadherin and β-catenin were localized. frizzled-3 was also localized in differentiating inner enamel epithelial cells. Vang11 was localized sparsely in differentiating preameloblasts and extensively at the cell boundary of stratum intermedium. Celsr1 was not localized in ameloblasts but localized in odontoblasts extensively. These results suggest the involvement of planar cell polarity proteins in odontogenesis.
使用免疫细胞化学技术检测了平面细胞极性蛋白 Vang12、frizzled-3、Vang11 和 Celsr1 在大鼠切牙中的定位。结果表明，Vang12 定位于大鼠切牙内釉腺分泌成釉细胞 Tomes' 突的两个区域：近端和远端区域。相比之下，frizzled-3 定位于内釉和外釉分泌成釉细胞的近端和远端区域的黏附连接点，N-钙黏蛋白和β-连环蛋白定位于此处。frizzled-3 也定位于分化中的内釉上皮细胞。Vang11 稀疏定位于分化中的前成釉细胞，广泛定位于中釉质层的细胞边界。Celsr1 不在成釉细胞中定位，而是广泛定位于成牙本质细胞中。这些结果表明平面细胞极性蛋白参与牙发生。
