Immunology Unit, Department of Physiology, University of Extremadura, Cáceres, Spain.
J Innate Immun. 2011;3(4):365-73. doi: 10.1159/000328505. Epub 2011 May 11.
The role of natural killer (NK) cells in tumor immunosurveillance has been recently underlined. A better understanding of the receptor-ligand interactions between NK cells and solid tumor cells is essential for introducing more effective NK cell-based immunotherapy protocols into clinical practice. We previously analyzed the surface expression of ligands for NK cell-activating receptors and costimulatory molecules in a large panel of melanoma cell lines. Although the expression of ligands for NK cell-activating receptors is variable, the majority of melanoma cell lines express ligands for NKG2D and for DNAX accessory molecule-1 (DNAM-1). While the NKG2D receptor has been described as the principal entity responsible for the lysis of several melanoma cell lines, the role of natural cytotoxicity receptors (NCRs) and DNAM-1 receptors in NK cell recognition and killing of melanoma cells has been recently emphasized. Antibody-mediated masking of NKG2D, NCRs, and DNAM-1 has proven that NKG2D, NCRs, and DNAM-1 frequently cooperate in the lysis of melanoma cells. In this work, we provide an overview of recent advances in the study of melanoma cells' susceptibility to NK cell-mediated lysis and how multiple receptor-ligand interactions participate in melanoma cell elimination.
自然杀伤 (NK) 细胞在肿瘤免疫监视中的作用最近受到了重视。为了将更有效的基于 NK 细胞的免疫治疗方案引入临床实践，深入了解 NK 细胞与实体瘤细胞之间的受体-配体相互作用至关重要。我们之前分析了大量黑色素瘤细胞系中 NK 细胞激活受体和共刺激分子的表面表达配体。尽管 NK 细胞激活受体的配体表达具有可变性，但大多数黑色素瘤细胞系表达 NKG2D 和 DNAX 辅助分子-1 (DNAM-1) 的配体。虽然 NKG2D 受体已被描述为导致几种黑色素瘤细胞系裂解的主要实体，但自然细胞毒性受体 (NCR) 和 DNAM-1 受体在 NK 细胞识别和杀伤黑色素瘤细胞中的作用最近受到了重视。抗体介导的 NKG2D、NCR 和 DNAM-1 的掩蔽已证明 NKG2D、NCR 和 DNAM-1 经常在黑色素瘤细胞的裂解中协同作用。在这项工作中，我们概述了黑色素瘤细胞对 NK 细胞介导的裂解敏感性研究的最新进展，以及多种受体-配体相互作用如何参与黑色素瘤细胞的消除。
