转移核糖核酸和蛋白质在2微米羟基磷灰石球形珠上的高效吸附色谱法。氯化钠和镁离子对分离度的影响。
High-performance adsorption chromatography of transfer ribonucleic acids and proteins on 2-microns spherical beads of hydroxyapatite. Influence of sodium chloride and magnesium ions on the resolution.
作者信息
Lindeberg J, Srichaiyo T, Hjertén S
机构信息
Institute of Biochemistry, University of Uppsala, Sweden.
出版信息
J Chromatogr. 1990 Jan 19;499:153-63. doi: 10.1016/s0021-9673(00)96970-x.
The influence of sodium chloride and magnesium chloride on the adsorption of tRNA and proteins on a high-performance liquid chromatographic column of 2-microns spherical hydroxyapatite beads was investigated. The resolution of 14C-labelled aminoacyl-tRNA isoacceptors was improved in the presence of sodium chloride. Inclusion of magnesium chloride in the buffers led to a separation of two tRNA species that could not be fractionated with or without sodium chloride in the eluting buffers (the original properties of the column were lost, however, and could not be regenerated by simply returning to magnesium chloride-free phosphate buffer). Also, the adsorption of some proteins was affected when salt was included in the buffers. For instance, the elution order of proteins could be changed by choosing an appropriate concentration of sodium chloride. This finding might be utilized to facilitate the purification of certain proteins.
研究了氯化钠和氯化镁对tRNA和蛋白质在2微米球形羟基磷灰石珠高效液相色谱柱上吸附的影响。在氯化钠存在的情况下，14C标记的氨酰-tRNA同工受体的分离度得到了提高。缓冲液中加入氯化镁导致两种tRNA物种的分离，而在洗脱缓冲液中无论有无氯化钠都无法对其进行分级分离（然而，柱的原始性质丧失，且不能通过简单地回到不含氯化镁的磷酸盐缓冲液中进行再生）。此外，当缓冲液中加入盐时，一些蛋白质的吸附也会受到影响。例如，通过选择合适浓度的氯化钠可以改变蛋白质的洗脱顺序。这一发现可能有助于某些蛋白质的纯化。
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