Miralles A, Olmos G, Ramírez G
Laboratorio de Biología Celular, Universidad de las Islas Baleares, Palma de Mallorca, Spain.
Neurochem Res. 1990 Jan;15(1):47-52. doi: 10.1007/BF00969183.
We have analyzed the developmental properties of kainate receptors in cerebellar membranes prepared from chick and rat, two vertebrate species with contrasting patterns of functional maturation. Single populations of binding sites have been characterized in the avian and rodent membranes with apparent dissociation constants (Kd) in the 210-280 nM and 40-55 nM ranges, respectively; the number of binding sites (Bmax) increases with age in both species, reaching a maximum of 187 pmol/mg in the case of 10-day chicks vs. 1.28 pmol/mg in 75-day rats. The ontogenetic profiles of kainate receptors in chick and rat cerebella are in consonance with the patent differences in motor development at birth.
我们分析了从小鸡和大鼠制备的小脑膜中红藻氨酸受体的发育特性，这两种脊椎动物具有截然不同的功能成熟模式。在鸟类和啮齿动物的膜中，已鉴定出单一的结合位点群体，其表观解离常数（Kd）分别在210 - 280 nM和40 - 55 nM范围内；两种物种的结合位点数（Bmax）均随年龄增加，10日龄小鸡的Bmax最高可达187 pmol/mg，而75日龄大鼠的Bmax为1.28 pmol/mg。小鸡和大鼠小脑的红藻氨酸受体个体发育概况与出生时运动发育的明显差异一致。
