Kumura K, Ibusuki K, Minamishima Y
Department of Microbiology, Miyazaki Medical College, Japan.
Virology. 1990 Apr;175(2):572-4. doi: 10.1016/0042-6822(90)90443-u.
A temperature-sensitive (ts) mutant of murine cytomegalovirus, ts21, lacks pathogenicity for suckling mice. Revertants (rev21) of ts21 which could replicate at nonpermissive temperature remained attenuated, suggesting the presence of a temperature-independent factor(s) for the attenuation. Coinfection of ts21 with its parental wild-type (wt) generated recombinant-type progenies (rec21w) which lacked temperature sensitivity and were still attenuated. The results indicate that ts21 harbors at least two mutations and the mutation(s) in pathogenicity exists independently of the ts mutation(s).
鼠巨细胞病毒的温度敏感（ts）突变体ts21对乳鼠无致病性。ts21的回复株（rev21）能够在非允许温度下复制，但仍表现出减毒特性，这表明存在一个与温度无关的减毒因子。ts21与其亲代野生型（wt）共同感染产生了缺乏温度敏感性且仍具减毒特性的重组型子代（rec21w）。结果表明，ts21至少含有两个突变，且致病性突变独立于ts突变存在。
