通过高碘酸盐反应及分光光度法或高效液相色谱法测定药品中的阿莫地喹。

Determination of amodiaquine in pharmaceuticals by reaction with periodate and spectrophotometry or by high-performance liquid chromatography.

作者信息

Sanghi S K, Verma A, Verma K K

机构信息

Department of Chemistry, Rani Durgavati University, Madhya Pradesh, India.

出版信息

Analyst. 1990 Mar;115(3):333-5. doi: 10.1039/an9901500333.

DOI:10.1039/an9901500333

PMID:2158252

Abstract

Two methods are described for the determination of amodiaquine in pharmaceuticals. In the first method, amodiaquine was treated with periodate at pH 7.5-8.5 to yield a chromogen, which is believed to be a 1,4-benzoquinone imine derivative, and extracted into chloroform for spectrophotometric assay. The maximum molar absorptivity was found to be 2.17 x 10(3) l mol-1 cm-1 at 442 nm. Primaquine and chloroquine, if present also, do not interfere. The second method, which was used for the simultaneous determination of amodiaquine, primaquine and chloroquine, is based on high-performance liquid chromatography on an octadecylsilane column using a mobile phase of methanol-water (80 + 20) containing 0.5% anhydrous acetic acid and an over-all concentration of sodium dodecyl sulphate of 0.5 mM, with detection at 340 nm.

摘要

文中描述了两种测定药品中阿莫地喹的方法。第一种方法是，在pH 7.5 - 8.5条件下，用高碘酸盐处理阿莫地喹以生成一种显色原，据信该显色原是一种1,4 - 苯醌亚胺衍生物，然后将其萃取到氯仿中进行分光光度测定。在442 nm处测得最大摩尔吸光系数为2.17×10³ l·mol⁻¹·cm⁻¹。如果同时存在伯氨喹和氯喹，它们不会产生干扰。第二种方法用于同时测定阿莫地喹、伯氨喹和氯喹，该方法基于在十八烷基硅烷柱上进行高效液相色谱分析，流动相为甲醇 - 水（80 + 20），其中含有0.5%的无水乙酸且十二烷基硫酸钠的总浓度为0.5 mM，检测波长为340 nm。