Sanghi S K, Verma A, Verma K K
Department of Chemistry, Rani Durgavati University, Madhya Pradesh, India.
Analyst. 1990 Mar;115(3):333-5. doi: 10.1039/an9901500333.
Two methods are described for the determination of amodiaquine in pharmaceuticals. In the first method, amodiaquine was treated with periodate at pH 7.5-8.5 to yield a chromogen, which is believed to be a 1,4-benzoquinone imine derivative, and extracted into chloroform for spectrophotometric assay. The maximum molar absorptivity was found to be 2.17 x 10(3) l mol-1 cm-1 at 442 nm. Primaquine and chloroquine, if present also, do not interfere. The second method, which was used for the simultaneous determination of amodiaquine, primaquine and chloroquine, is based on high-performance liquid chromatography on an octadecylsilane column using a mobile phase of methanol-water (80 + 20) containing 0.5% anhydrous acetic acid and an over-all concentration of sodium dodecyl sulphate of 0.5 mM, with detection at 340 nm.
文中描述了两种测定药品中阿莫地喹的方法。第一种方法是，在pH 7.5 - 8.5条件下，用高碘酸盐处理阿莫地喹以生成一种显色原，据信该显色原是一种1,4 - 苯醌亚胺衍生物，然后将其萃取到氯仿中进行分光光度测定。在442 nm处测得最大摩尔吸光系数为2.17×10³ l·mol⁻¹·cm⁻¹。如果同时存在伯氨喹和氯喹，它们不会产生干扰。第二种方法用于同时测定阿莫地喹、伯氨喹和氯喹，该方法基于在十八烷基硅烷柱上进行高效液相色谱分析，流动相为甲醇 - 水（80 + 20），其中含有0.5%的无水乙酸且十二烷基硫酸钠的总浓度为0.5 mM，检测波长为340 nm。
