Sheng W W, Soukup S, Bove K, Gotwals B, Lampkin B
Department of Human Genetics, Children's Hospital Medical Center, Cincinnati, Ohio 45229.
Cancer Res. 1990 May 1;50(9):2786-93.
Cytogenetic analysis was done on 31 Wilms' tumors, including 2 renal tumors of clear cell sarcoma type, using short term cultures of primary tumors and/or nude mouse passages. Nonrandom secondary chromosome abnormalities, in particular, were noted as evidence of clonal evolution. Apparently normal karyotypes were found in 5 Wilms' tumors, all in patients less than or equal to 22 months old, and in one clear cell sarcoma. Abnormal karyotypes were seen in 25 tumors (80%); 6 were pseudodiploid, 3 were hypodiploid, and 16 (52%) were hyperdiploid, of which 8 had a modal number of 47-49 and 8 had a modal number of 50-55. Nonrandom structural abnormalities involved 1p/1q, 11p, 7p/7q, 16p/16q, 12q, and 17p/17q. Nonrandom numerical abnormalities included +6, +8, and +18. Trisomy 12 was the most common abnormality, structural or numerical, seen in 52% of tumors (81% of the hyperdiploid). In 2 tumors the +12 was the only apparent abnormality; in 1 other tumor an i(12q) was seen, suggesting that +12 may have special significance in the clonal progression of Wilms' tumor. Informative karyotypes of 68 Wilms' tumors from other reports were reviewed and compared to results in this series.
对31例肾母细胞瘤进行了细胞遗传学分析，其中包括2例透明细胞肉瘤型肾肿瘤，采用原代肿瘤短期培养和/或裸鼠传代培养。特别值得注意的是，非随机继发性染色体异常被视为克隆进化的证据。在5例肾母细胞瘤中发现了明显正常的核型，所有这些病例的患者年龄均小于或等于22个月，另外在1例透明细胞肉瘤中也发现了正常核型。在25例肿瘤（80%）中观察到异常核型；6例为假二倍体，3例为亚二倍体，16例（52%）为超二倍体，其中8例众数为47 - 49，8例众数为50 - 55。非随机结构异常涉及1p/1q、11p、7p/7q、16p/16q、12q和17p/17q。非随机数量异常包括 +6、+8和 +18。12号染色体三体是最常见的异常，包括结构异常或数量异常，在52%的肿瘤中出现（超二倍体肿瘤中的81%）。在2例肿瘤中，+12是唯一明显的异常；在另一例肿瘤中观察到i(12q)，这表明 +12可能在肾母细胞瘤的克隆进展中具有特殊意义。回顾了其他报告中68例肾母细胞瘤的信息性核型，并与本系列结果进行了比较。
