Silvestrini R, Veneroni S, Benini E, Difronzo G, Daidone M
CNR,CTR STUDIO PATOL CELLULARE,I-20133 MILAN,ITALY.
Int J Oncol. 1992 Oct;1(5):507-12. doi: 10.3892/ijo.1.5.507.
p53 and cathepsin D expression was investigated in 300 primary breast cancers by the avidin-biotin immunoperoxidase method using two murine monoclonal antibodies: PAb1801 and anti-procathepsin D, respectively. The frequency of p53- and cathepsin D-positive cells varied widely among different tumors and most tumors (82% and 86%) at least occasionally showed positive cells. The two biological markers were unrelated to one another, to cell proliferative rate and ploidy and were differently related to other biological and pathological features. In particular, p53 was directly related to tumor size and nodal involvement and inversely related to the presence of steroid receptors. Conversely, cathepsin D was directly related only to nodal involvement.
采用抗生物素蛋白-生物素免疫过氧化物酶法，分别使用两种鼠单克隆抗体（PAb1801和抗组织蛋白酶D），对300例原发性乳腺癌中的p53和组织蛋白酶D表达进行了研究。不同肿瘤中p53和组织蛋白酶D阳性细胞的频率差异很大，大多数肿瘤（82%和86%）至少偶尔会出现阳性细胞。这两种生物学标志物彼此无关，与细胞增殖率和倍体也无关，且与其他生物学和病理特征的相关性也不同。特别是，p53与肿瘤大小和淋巴结受累直接相关，与类固醇受体的存在呈负相关。相反，组织蛋白酶D仅与淋巴结受累直接相关。
