Firon N, Schejter A
Sackler Institute of Molecular Medicine, Sackler Faculty of Medicine, Tel Aviv University, Israel.
Exp Cell Res. 1990 May;188(1):10-5. doi: 10.1016/0014-4827(90)90271-b.
The system responsible for the oxidative burst (OB) activity in Paracentrotus lividus eggs is different from those described earlier for other sea urchin species. The OB in P. lividus is associated with a soluble fraction resulting from centrifugation at 150,000 g. A low-molecular-weight, -SH-containing molecule present in this supernatant is required for the OB activity. The sulfhydryl reagent N-ethylmaleimide completely inhibits the calcium stimulated OB activity of intact eggs in the presence of the calcium ionophore A23187, suggesting that this requirement of low-molecular-weight -SH-containing molecules for OB exists also in vivo.
紫海胆卵中负责氧化爆发（OB）活性的系统与之前描述的其他海胆物种的系统不同。紫海胆中的OB与150,000 g离心产生的可溶性部分相关。该上清液中存在的一种低分子量、含-SH的分子是OB活性所必需的。巯基试剂N-乙基马来酰亚胺在钙离子载体A23187存在的情况下完全抑制完整卵的钙刺激OB活性，这表明体内也存在对低分子量含-SH分子的这种OB需求。
