口腔鳞状细胞癌中mam-3和mam-6抗原的免疫组织化学评估。

Immunohistochemical evaluation of mam-3 and mam-6 antigens in squamous-cell carcinoma of the oral cavity.

作者信息

Kunikata M, Yamada K, Ogata K, Mori M, Tatemoto Y, Osaki T, Tubura A, Morii S

机构信息

ASAHI UNIV,SCH DENT,DEPT ORAL & MAXILLOFACIAL SURG,1851-1 HOZUMI CHO,MOTOSU,GIFU 50102,JAPAN. KANSAI MED UNIV,DEPT PATHOL,MORIGUCHI,OSAKA 570,JAPAN. KOCHI MED SCH,DEPT ORAL SURG,NANKOKU,KOCHI 78151,JAPAN.

出版信息

Int J Oncol. 1992 Dec;1(7):753-8.

PMID:21584612

Abstract

MAM-3 and MAM-6 antigens of human milk fat globule membrane designated as human polymorphic epithelial mucin (PEM) were detected immunohistochemically in a total of 86 specimens from 39 cases of oral squamous cell carcinoma (SCC) and 6 cases of normal oral mucosa. The two antigens were identified by MoAbs 67D11 and 115G3 (MAM-3 antigen) and by MoAbs 115D8 and 115F5 (MAM-6 antigen) in paraffin sections. MoAb 115G3 staining was found in well keratinized foci; whereas, MoAb 115D8 and 115F5 staining was very low in keratinized tumor cells. The cytochemical distribution of the antigens was classified into 3 types; (i) cell membrane positive type, (ii) whole cell positive type and (iii) antigen negative type. Reduction and disappearance of the two antigens were correlated to the mode of tumor invasion and suggested that glycocalyx complex in cell membrane properties was changed for malignant transformation.

摘要

在39例口腔鳞状细胞癌（SCC）和6例正常口腔黏膜的总共86个标本中，通过免疫组织化学检测了人乳脂肪球膜中被指定为人多形性上皮粘蛋白（PEM）的MAM - 3和MAM - 6抗原。在石蜡切片中，两种抗原分别通过单克隆抗体67D11和115G3（MAM - 3抗原）以及单克隆抗体115D8和115F5（MAM - 6抗原）进行鉴定。发现单克隆抗体115G3染色出现在角化良好的病灶中；而单克隆抗体115D8和115F5在角化肿瘤细胞中的染色非常低。抗原的细胞化学分布分为3种类型：（i）细胞膜阳性型，（ii）全细胞阳性型和（iii）抗原阴性型。这两种抗原的减少和消失与肿瘤侵袭方式相关，提示细胞膜特性中的糖萼复合物因恶性转化而发生改变。