[基于实时聚合酶链反应法的炭疽病原体DNA检测分子生物学检测系统的研发与测试]

[The development and testing of a molecular biological test system for DNA detection of anthrax pathogen by real-time polymerase chain reaction assay].

作者信息

Abramov D D, Vorob'ev A A, Kuznetsovskiĭ A V, Savinykh A V, Onuchina N V, Darmov I V, Trofimov D Iu, Kuznetsov S L

出版信息

Klin Lab Diagn. 2011 Mar(3):46-50.

Abstract

The paper presents the results of the development and testing of a molecular biological test system for DNA detection of anthrax pathogen (Bacillus anthracis) by real-time polymerase chain reaction assay. The test system has shown high sensitivity, specificity, and reproducibility of results of analysis, as exemplified by aqueous suspensions of daily agar cultures of Bacillus anthracis strains, related and heterologous species of microorganisms, and clinical materials of experimental animals. There is evidence for the persistence of the basic characteristics of the test system when stored at 22 +/- 2 degrees C for 12 months.

摘要

本文介绍了一种用于通过实时聚合酶链反应测定法检测炭疽病原体（炭疽芽孢杆菌）DNA的分子生物学检测系统的开发和测试结果。该检测系统已显示出分析结果具有高灵敏度、特异性和可重复性，炭疽芽孢杆菌菌株的日常琼脂培养物的水悬浮液、相关和异源微生物物种以及实验动物的临床材料均可作为例证。有证据表明，该检测系统在22±2摄氏度下储存12个月时，其基本特性仍然存在。