Xu Rong, Zhu Weicheng, Chen Jun, Wang Xia, Liu Tongning
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Beijing 100193, China.
Zhongguo Zhong Yao Za Zhi. 2011 Feb;36(3):307-10.
To study the characteristics of pollen viability and stigma receptivity of C. deserticola and provide theory basis for seed production and breeding of C. deserticola.
Different physiological measurement methods were applied to evaluate pollen viability and stigma receptivity. The results of different methods were compared with the seed setting percentage of the cross-pollination in the field test and pollen germination percentage by fluoroscope observation methods. The changes of pollen vitality and stigma receptivity in different conditions were tested using proper methods.
The optimum methods on pollen viability and stigma receptivity detection were MTT-test and Benzidine-Hydrogen Peroxide method respectively. Results showed that the mean pollen viability and stigma receptivity were both the highest in inchoate anthesis with pollen germination percentage up to 95%, and can maintain viable for 4-5 d, but at the lower temperature of 4 degrees C, the pollen can be stored up to 10 days.
The physiological characteristics of pollen and stigma of C. deserticola displayed good ecological adaptation, which are much more adaptive to the large area of cultivation condition.
研究肉苁蓉花粉活力和柱头可授性的特点，为肉苁蓉种子生产和育种提供理论依据。
采用不同的生理测定方法评估花粉活力和柱头可授性。将不同方法的结果与田间试验异花授粉的结实率以及荧光显微镜观察法的花粉萌发率进行比较。使用适当的方法检测不同条件下花粉活力和柱头可授性的变化。
检测花粉活力和柱头可授性的最佳方法分别是MTT法和联苯胺-过氧化氢法。结果表明，在初花期花粉活力和柱头可授性均值均最高，花粉萌发率高达95%，且能保持活力4 - 5天，但在4℃较低温度下，花粉可保存长达10天。
肉苁蓉花粉和柱头的生理特性表现出良好的生态适应性，更适应大面积栽培条件。
