Department of Bioengineering and Bioinformatics, Lomonosov Moscow State University, Russia.
Biochemistry (Mosc). 2011 Apr;76(4):491-2. doi: 10.1134/s0006297911040134.
The possible application of the quasi-equilibrium assumption for an arbitrary mechanism of enzymatic reaction is considered. It is shown at what ratios of kinetic constants a segment consisting of two, three, and four intermediates may be considered as an equilibrium one. Expressions for evaluation of accuracy of distribution of intermediate concentrations inside the equilibrium segment and accuracy of determination of intermediate concentrations inside and outside the equilibrium segment as a function of the ratio of kinetic constants are derived. A method for determination of the limitations on the ratio of rate constants for an equilibrium segment of arbitrary structure is suggested.
本文考虑了准平衡假设在任意酶反应机制中的可能应用。文中给出了在何种动力学常数比值下，由两个、三个和四个中间物组成的片段可以被视为平衡片段。文中还推导出了用于评估平衡片段内中间物浓度分布的准确性以及确定平衡片段内外中间物浓度准确性的表达式，这些表达式是作为动力学常数比值的函数。文中提出了一种用于确定任意结构的平衡片段的速率常数比值限制的方法。
