Laboratory of Experimental Bacteriology, National Institute of Pediatrics, Mexico City, Mexico.
Helicobacter. 2011 Jun;16(3):200-9. doi: 10.1111/j.1523-5378.2011.00831.x.
Mongolian gerbils that are experimentally infected with Helicobacter pylori develop a chronic inflammation that is similar to natural infections in humans. The aim of this study was to compare the antigens of H. pylori cagPAI+ and cagPAI- strains that are expressed during Meriones unguiculatus colonization.
We identified H. pylori cagPAI+ and cagPAI- strain antigens via Western blotting of samples from Mongolian gerbils that were subjected to unique, mixed, and sequential bacterial infections.
The antigens from the J99/CG3 (cagPAI+) strain had a lower molecular weight than the antigens from the 251F/CG3 (cagPAI-) strain. There were fewer identified antigens in the single unique infections compared with the mixed and sequential infections. The number of recognized antigens that had a frequency of recognition >60% was higher for the simultaneous and sequential infection groups compared with the single infection group. A 57-kDa antigen was present in >60% of the samples and four of the five experimental groups. Antigens specific to each bacterial strain were identified; the 190- and 158-kDa antigens appear to be specific for cagPAI-, and the 70-kDa antigen appears to be specific for cagPAI+.
In this study, we identified antigens that are common and specific to the H. pylori cagPAI+ and cagPAI- strains.
实验感染幽门螺旋杆菌的蒙古沙鼠会发展出类似于人类自然感染的慢性炎症。本研究旨在比较在蒙古沙鼠定植过程中表达的 cagPAI+和 cagPAI-幽门螺旋杆菌菌株的抗原。
我们通过对经历独特、混合和连续细菌感染的蒙古沙鼠样本进行 Western 印迹，鉴定了 H. pylori cagPAI+和 cagPAI-菌株的抗原。
J99/CG3（cagPAI+）菌株的抗原分子量低于 251F/CG3（cagPAI-）菌株。与混合和连续感染相比，单一独特感染中鉴定出的抗原较少。识别频率>60%的识别抗原数量在同时和连续感染组中高于单一感染组。>60%的样本和五个实验组中的四个都存在 57-kDa 抗原。鉴定出了针对每种细菌株的特异性抗原；190-和 158-kDa 抗原似乎特异性针对 cagPAI-，而 70-kDa 抗原似乎特异性针对 cagPAI+。
在这项研究中，我们鉴定了 cagPAI+和 cagPAI-幽门螺旋杆菌菌株的共同和特异性抗原。
