Alfred-Wegener-Institute for Polar and Marine Research, Am Handelshafen 12, 27570 Bremerhaven, Germany, Urnenfelderstraße 9, 85051 Ingolstadt, Germany.
Mol Ecol Resour. 2008 Jan;8(1):99-102. doi: 10.1111/j.1471-8286.2007.01927.x.
The recent introduction of phylochips that contain molecular probes facilitates environmental microbial identification in a single experiment without previous cultivation. A set of probes recognizing species at different taxonomic levels is denoted as a hierarchical set. Application of hierarchical probe sets on a DNA microarray allows the assessment of biodiversity with different resolutions. It significantly increases the robustness of the results retrieved from phylochip experiments because of the possible consistency checks of hybridization across different taxonomic levels. Here, we present a computer program, phylo-chipanalyser, for the hierarchy editing and the evaluation of phylochip data generated from hierarchical probe sets.
最近引入的 phylochips 包含分子探针，可在无需事先培养的情况下，在单次实验中促进环境微生物的鉴定。一组识别不同分类学水平的物种的探针称为层次探针组。在 DNA 微阵列上应用层次探针组可以以不同的分辨率评估生物多样性。由于可能在不同的分类学水平上进行杂交的一致性检查，这极大地提高了从 phylochip 实验中获得的结果的稳健性。在这里，我们介绍了一个计算机程序 phylo-chipanalyser，用于层次编辑和评估来自层次探针组的 phylochip 数据。
