开发和描述来自深海热液喷口硫细菌共生体 Riftia pachyptila 的 12 个微卫星标记。

Development and characterization of 12 microsatellite markers from the deep-sea hydrothermal vent siboglinid Riftia pachyptila.

机构信息

Biology Department, MS#33, Redfield Laboratory, Woods Hole Oceanographic Institution, Woods Hole, MA 02543, USA, Marine Biological Laboratory, 7 MBL Street, Woods Hole, MA 02543, USA.

出版信息

Mol Ecol Resour. 2008 Jan;8(1):132-4. doi: 10.1111/j.1471-8286.2007.01897.x.

DOI:10.1111/j.1471-8286.2007.01897.x

PMID:21585736

Abstract

Ecological processes at deep-sea hydrothermal vents on fast-spreading mid-ocean ridges are punctuated by frequent physical disturbance, often accompanied by a high occurrence of population turnover. To persist through local extinction events, sessile invertebrate species living in these geologically and chemically dynamic habitats depend on larval dispersal. We characterized 12 polymorphic microsatellite loci from one such species, the siboglinid tubeworm Riftia pachyptila. All loci conformed to Hardy-Weinberg expectations without linkage (mean H(E) = 0.9405, mean N(A) = 20.25). These microsatellites are being employed in the investigation of spatial and temporal population genetic structure in the eastern Pacific Ocean.

摘要

深海热液喷口的生态过程受到频繁的物理干扰，这些干扰通常伴随着种群更替的高发。为了在局部灭绝事件中生存下来，生活在这些地质和化学动态生境中的固着无脊椎动物物种依赖于幼虫的扩散。我们从一种这样的物种，即裂唇蛤科管虫 Riftia pachyptila 中鉴定了 12 个多态性微卫星位点。所有位点均符合哈迪-温伯格期望，没有连锁（平均 H(E) = 0.9405，平均 N(A) = 20.25）。这些微卫星正在被用于调查东太平洋的空间和时间种群遗传结构。