永久性遗传资源：异腹胡蜂（膜翅目：长尾小胡蜂科）的多态微卫星位点。

PERMANENT GENETIC RESOURCES: Polymorphic microsatellite loci for Diachasma alloeum (Hymenoptera: Braconidae).

机构信息

Department of Biological Sciences, 107 Galvin Life Science Building, University of Notre Dame, Notre Dame, IN 46556, USA, Biology Department, Duke University, Research Drive, LSRC B333, Box 90338, Durham, NC 27708, USA.

出版信息

Mol Ecol Resour. 2008 Mar;8(2):373-6. doi: 10.1111/j.1471-8286.2007.01961.x.

DOI:10.1111/j.1471-8286.2007.01961.x

PMID:21585796

Abstract

Here, we report the isolation of 21 novel primers for amplification of microsatellite loci in Diachasma alloeum (Hymenoptera: Braconidae). Diachasma alloeum is a larval parasitoid of the apple maggot fly (Rhagoletis pomonella), which is an economically significant agricultural pest species and a textbook example of sympatric speciation via host-plant shifting. These microsatellite markers will prove useful both for assessing genetic relationships between different host-associated populations of D. alloeum, as well as for future R. pomonella biological control programmes. We also report the cross-species amplification of several loci for Diachasmimorpha mellea and Diachasma ferrugineum, parasitoids of R. pomonella and R. cingulata, respectively.

摘要

在这里，我们报告了 21 个新的引物的分离，用于扩增 Diachasma alloeum（膜翅目：Braconidae）中的微卫星位点。Diachasma alloeum 是苹果小卷叶蛾（Rhagoletis pomonella）的幼虫寄生蜂，是一种经济上重要的农业害虫物种，也是通过寄主植物转移实现同域物种形成的经典范例。这些微卫星标记物将证明对评估不同寄主相关种群之间的遗传关系非常有用，同时也对未来的苹果小卷叶蛾生物防治计划非常有用。我们还报告了几个位点的跨物种扩增，这些位点分别来自 Rhagoletis pomonella 和 R. cingulata 的寄生蜂 Diachasmimorpha mellea 和 Diachasmiferrugineum。