Jenkins Cheryl, Chapman Toni A, Micallef Jessica L, Reynolds Olivia L
Elizabeth Macarthur Agricultural Institute, NSW Department of Primary Industries, Woodbridge Road, Menangle, NSW 2568, Australia.
Insects. 2012 Aug 14;3(3):763-88. doi: 10.3390/insects3030763.
Parasitoid detection and identification is a necessary step in the development and implementation of fruit fly biological control strategies employing parasitoid augmentive release. In recent years, DNA-based methods have been used to identify natural enemies of pest species where morphological differentiation is problematic. Molecular techniques also offer a considerable advantage over traditional morphological methods of fruit fly and parasitoid discrimination as well as within-host parasitoid identification, which currently relies on dissection of immature parasitoids from the host, or lengthy and labour-intensive rearing methods. Here we review recent research focusing on the use of molecular strategies for fruit fly and parasitoid detection and differentiation and discuss the implications of these studies on fruit fly management.
寄生蜂的检测与鉴定是采用寄生蜂增殖释放的果蝇生物防治策略开发与实施过程中的必要步骤。近年来，在形态分化存在问题的情况下，基于DNA的方法已被用于识别害虫物种的天敌。与传统的果蝇和寄生蜂鉴别形态学方法以及目前依赖于从宿主中解剖未成熟寄生蜂或冗长且劳动密集型饲养方法的宿主内寄生蜂鉴定方法相比，分子技术也具有相当大的优势。在此，我们综述了近期聚焦于利用分子策略进行果蝇和寄生蜂检测与鉴别的研究，并讨论了这些研究对果蝇管理的影响。
