School of Natural Sciences, University of California, Merced, PO Box 2039, Merced, CA 95340, USA, NOAA Southwest Fisheries Science Center, 110 Shaffer Road, Santa Cruz, CA 95060, USA, Institute of Marine Sciences, University of California, Santa Cruz, 110 Shaffer Road, Santa Cruz, CA 95060, USA.
Mol Ecol Resour. 2008 May;8(3):659-62. doi: 10.1111/j.1471-8286.2007.02038.x.
We describe 15 single nucleotide polymorphisms (SNPs) isolated in coastal California populations of steelhead (Oncorhynchus mykiss). SNP loci were developed using a 'gene-targeted' approach, which involved the development of primers from functional genes in O. mykiss that were deposited in GenBank or in the published literature. These markers show a wide range of variability in three coastal steelhead populations, and will be useful in population genetic studies and in pedigree reconstruction. Potential applications include evaluation of population structure, introgression between native and hatchery trout, and evaluating reproductive success.
我们描述了在加利福尼亚沿海地区的虹鳟鱼种群中分离出的 15 个单核苷酸多态性（SNP）。SNP 基因座是使用“基因靶向”方法开发的，该方法涉及从虹鳟鱼的功能基因中开发引物，这些基因已存入 GenBank 或已发表的文献中。这些标记在三个沿海虹鳟鱼群体中显示出广泛的变异性，并且将在群体遗传研究和系谱重建中非常有用。潜在的应用包括评估种群结构、本土和孵化场鳟鱼之间的基因渗入以及评估繁殖成功率。
