一种用于鉴定儒艮（Dugong dugon）和西印度海牛（Trichechus manatus）性别的 PCR 检测方法。
A PCR assay for gender assignment in dugong (Dugong dugon) and West Indian manatee (Trichechus manatus).
机构信息
Molecular Fisheries Laboratory, Queensland Department of Primary Industries and Fisheries, Queensland Biosciences Precinct, University of Queensland, Level 6 North Tower, 306 Carmody Road, St Lucia, Qld 4072, Australia, School of Integrative Biology, University of Queensland, St Lucia, Qld 4072, Australia.
出版信息
Mol Ecol Resour. 2008 May;8(3):669-70. doi: 10.1111/j.1471-8286.2007.02041.x.
Gender assignment for some aquatic mammals in the field is difficult. Molecular sexing from tissue biopsies is possible as males are heterogametic. Here we describe a multiplex PCR assay that amplifies the male specific SRY gene and differentiates ZFX and ZFY gametologues in two sirenian species, dugong (Dugong dugon) and West Indian manatee (Trichechus manatus). The assay was validated with animals of known gender and proved accurate and robust to experimental failure.
一些水生哺乳动物的性别鉴定在野外较为困难。通过组织活检进行分子性别鉴定是可行的，因为雄性是异型性别的。本文描述了一种多重 PCR 检测方法，可扩增雄性特有的 SRY 基因，并区分两种海牛物种（儒艮和西印度海牛）的 ZFX 和 ZFY 同源基因。该检测方法在已知性别的动物中得到了验证，结果准确且稳健，不受实验失败的影响。
Zotero 插件