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黄杨木属(蒺藜科)中多态性微卫星位点的分离、鉴定和交叉扩增。

Isolation, characterization, and cross-amplification of polymorphic microsatellite loci in Guaiacum coulteri (Zygophyllaceae).

机构信息

Centro de Investigaciones en Ecosistemas, Universidad Nacional Autónoma de México, Antigua Carretera a Pátzcuaro no. 8701, Col. Ex-Hacienda de San José de la Huerta, Morelia, 58190 Michoacán, México.

出版信息

Mol Ecol Resour. 2008 May;8(3):671-4. doi: 10.1111/j.1471-8286.2007.02043.x.

DOI:10.1111/j.1471-8286.2007.02043.x
PMID:21585867
Abstract

Guaiacum coulteri is a dry forest hardwood species of conservation concern endemic to the Pacific coast of Mexico. Fifteen microsatellite markers were developed which show high levels of polymorphism across two populations with the number of alleles ranging from four to 21. Most loci additionally exhibited consistent multiple banding patterns, indicating the likely polyploidy of this species. All loci were tested for cross-amplification with most found to amplify well across the genus Guaiacum, although amplification in other related genera of the Zygophyllaceae was limited.

摘要

圭亚那苦木是一种干燥森林硬木物种,受到保护,是墨西哥太平洋海岸特有的。开发了 15 个微卫星标记,这些标记在两个种群中表现出高度多态性,等位基因数从 4 到 21 不等。大多数位点还表现出一致的多带模式,表明该物种可能是多倍体。所有位点都进行了交叉扩增测试,大多数位点在圭亚那属中扩增良好,尽管在茄科的其他相关属中的扩增受到限制。

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