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受威胁特有种 Mammillaria crucigera(仙人掌科)的多态性微卫星位点的分离、鉴定及交叉扩增。

Isolation, characterization and cross-amplification of polymorphic microsatellite loci in the threatened endemic Mammillaria crucigera (Cactaceae).

机构信息

Unidad de Biología, Tecnología y Prototipos, Facultad de Estudios Superiores Iztacala, Universidad Nacional Autónoma de México, Av. Barrios 1. Los Reyes Iztacala, Tlalnepantla, 54090 Estado de México, México, CIEco, Universidad Nacional Autónoma de México, Antigua carretera a Pátzcuaro, 8701 San José la Huerta, Morelia, 58190 Michoacán, México.

出版信息

Mol Ecol Resour. 2009 Jan;9(1):156-8. doi: 10.1111/j.1755-0998.2008.02422.x. Epub 2008 Oct 13.

DOI:10.1111/j.1755-0998.2008.02422.x
PMID:21564589
Abstract

The cactus Mammillaria crucigera is a threatened species endemic to central Mexico. As a means of assessing population genetic status of these species, eight microsatellite markers were developed. These primers were tested in 40 individual from two wild populations. The results showed that these primers will be useful to describe population structure and aid to the conservation of species. The eight primers were tested in other Mammillaria species and most of them showed successful amplification.

摘要

柱状仙人掌 Mammillaria crucigera 是一种分布于中美洲的濒危物种。为了评估这些物种的种群遗传状况,开发了 8 个微卫星标记。这些引物在来自两个野生种群的 40 个个体中进行了测试。结果表明,这些引物将有助于描述种群结构,并有助于物种保护。这 8 个引物在其他柱状仙人掌物种中进行了测试,其中大多数都成功扩增。

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