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北方玉米根萤叶甲和墨西哥玉米根萤叶甲(昆虫纲:鞘翅目:叶甲科)多态微卫星位点的开发及与其他 Diabrotica spp. 的交叉扩增

Polymorphic microsatellite loci from northern and Mexican corn rootworms (Insecta: Coleoptera: Chrysomelidae) and cross-amplification with other Diabrotica spp.

机构信息

Molecular Ecology Research Branch, National Exposure Research Laboratory, US Environmental Protection Agency, 26 W. Martin Luther King Drive, Cincinnati, OH 45268, USA.

出版信息

Mol Ecol Resour. 2008 May;8(3):707-9. doi: 10.1111/j.1471-8286.2007.02056.x.

DOI:10.1111/j.1471-8286.2007.02056.x
PMID:21585878
Abstract

The northern corn rootworm (Diabrotica barberi) and Mexican corn rootworm (Diabrotica virgifera zeae) are significant agricultural pests. For the northern corn rootworm, and to a lesser extent, the Mexican corn rootworm, high resolution molecular markers are needed. Here we present 14 polymorphic microsatellite loci isolated from libraries constructed using pooled northern and Mexican corn rootworm genomic DNA. Polymorphism in other Diabrotica, including the banded cucumber beetle, southern corn rootworm and western corn rootworm, is described.

摘要

北方玉米根萤叶甲(Diabrotica barberi)和墨西哥玉米根萤叶甲(Diabrotica virgifera zeae)是重要的农业害虫。对于北方玉米根萤叶甲,以及在较小程度上,墨西哥玉米根萤叶甲,需要高分辨率的分子标记。在这里,我们从使用北方和墨西哥玉米根萤叶甲基因组 DNA 构建的混合文库中分离出了 14 个多态性微卫星标记。还描述了其他 Diabrotica 物种(包括带条黄瓜叶甲、南方玉米根萤叶甲和西部玉米根萤叶甲)的多态性。

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