开发新的微卫星基因座和多重反应体系用于美洲白鲟（Esox masquinongy）。

Development of new microsatellite loci and multiplex reactions for muskellunge (Esox masquinongy).

机构信息

US Geological Survey, Wisconsin Cooperative Fishery Research Unit, College of Natural Resources, 800 Reserve Street, University of Wisconsin-Stevens Point, WI 54481, USA.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):811-3. doi: 10.1111/j.1755-0998.2007.02075.x.

DOI:10.1111/j.1755-0998.2007.02075.x

PMID:21585899

Abstract

The muskellunge (Esox masquinongy) is a valued fisheries species throughout its native range. Numerous studies have documented performance and phenotypic differences among muskellunge populations, but genetic markers for assessment have been lacking. We characterized 14 microsatellite loci and developed five multiplex polymerase chain reactions. Successful amplification of northern pike (Esox lucius) was observed for seven loci. These microsatellites will be useful for analysing population structure, performance characteristics of propagated strains, and helping to develop and monitor hatchery management guidelines for muskellunge.

摘要

棘胸蛙（Esox masquinongy）是其原生范围内具有重要渔业价值的物种。许多研究记录了棘胸蛙种群之间的表现和表型差异，但缺乏评估的遗传标记。我们对 14 个微卫星基因座进行了特征分析，并开发了 5 种多重聚合酶链反应。在 7 个基因座中观察到了北方狗鱼（Esox lucius）的成功扩增。这些微卫星将有助于分析种群结构、繁殖种群的表现特征，并有助于制定和监测棘胸蛙的孵化场管理指南。