乳木果树（Vitellaria paradoxa C. F. Gaertn）新型微卫星标记的分离与特征分析。
Isolation and characterization of new microsatellite markers in shea tree (Vitellaria paradoxa C. F. Gaertn).
机构信息
CIRAD - BIOS, Unité Propre de Recherche de Génétique Forestière, Campus international de Baillarguet TA A-39/C, 34398 Montpellier Cedex 5, France, IER (Institut d'Economie Rurale), Programme Ressources Forestières, Centre Régional de la Recherche Agronomique de Sotuba, BP 258, Bamako, Mali, IER (Institut d'Economie Rurale), Programme Ressources Forestières, Centre Régional de la Recherche Agronomique de Sikasso, BP 178, Sikasso, Mali.
出版信息
Mol Ecol Resour. 2008 Jul;8(4):822-4. doi: 10.1111/j.1755-0998.2007.02079.x.
Vitellaria paradoxa is one of the major components of African parkland agroforestry systems. In order to assess the genetic diversity and population structure of this species, we isolated and characterized 14 polymorphic nuclear microsatellite loci. Primers developed to amplify these loci were used to analyse 200 individuals of a shea tree population in Mali. Loci have shown a high number of alleles ranging from four to 26, and display an observed level of heterozygosity between 0.37 and 0.85. These new very polymorphic microsatellite markers will be useful for genetic and ecological studies of V. paradoxa.
奶茄是非洲热带稀树草原农林复合系统的主要组成部分之一。为了评估该物种的遗传多样性和种群结构，我们分离并鉴定了 14 个多态性核微卫星位点。用于扩增这些位点的引物被用于分析马里一个乳木果树种群的 200 个个体。这些位点显示出从 4 到 26 个等位基因的高度多态性，并且显示出观测到的杂合度水平在 0.37 到 0.85 之间。这些新的非常多态性微卫星标记将有助于对奶茄的遗传和生态研究。
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