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从濒危的新西兰几维鸟(鹤形目;秧鸡科;大斑秧鸡)中分离和特征化微卫星位点。

Isolation and characterization of microsatellite loci from the endangered New Zealand takahe (Gruiformes; Rallidae; Porphyrio hochstetteri).

机构信息

Department of Zoology, University of Otago, PO Box 56, Dunedin, New Zealand.

出版信息

Mol Ecol Resour. 2008 Jul;8(4):884-6. doi: 10.1111/j.1755-0998.2008.02098.x.

DOI:10.1111/j.1755-0998.2008.02098.x
PMID:21585919
Abstract

Nineteen polymorphic microsatellite loci were characterized from the endangered takahe (Porphyrio hochstetteri). Like many of New Zealand's other native avian species, levels of polymorphism were low, with variation detected at only 19 of 110 (17.3%) loci, and most polymorphic loci (78.9%) were diallelic (mean number of alleles = 2.3). Despite these low levels of variation, the microsatellites developed here will be useful for parentage assignment for confirming pedigrees, and investigating relationships between genetic variation, pedigree-based inbreeding and reproductive success in this highly endangered species.

摘要

从濒危物种大斑几维鸟(Porphyrio hochstetteri)中鉴定出了 19 个多态性微卫星位点。与新西兰的许多其他本地鸟类物种一样,大斑几维鸟的遗传多态性水平较低,在 110 个位点中仅检测到 19 个(17.3%)多态性位点,大多数多态性位点(78.9%)为二态性(平均等位基因数=2.3)。尽管这种变异水平较低,但这里开发的微卫星将有助于亲子关系的鉴定,以确认谱系,并研究这种高度濒危物种的遗传变异、基于谱系的近亲繁殖和繁殖成功率之间的关系。

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