Course in Ecological Genetics, Graduate School of Environmental Science, Hokkaido University, Sapporo 060-0810, Japan.
Mol Ecol Resour. 2008 Sep;8(5):996-7. doi: 10.1111/j.1755-0998.2008.02133.x. Epub 2008 Jun 28.
We developed 11 polymorphic microsatellite [simple sequence repeat (SSR)] loci from genomic DNA of Veratrum album ssp. oxysepalum using a dual-suppression polymerase chain reaction technique and an improved method. These markers, with four to 17 alleles per locus, identified 47 genotypes in 48 samples collected from a population in Hokkaido, Japan. The observed and expected heterozygosities ranged from 0.042 to 0.938 and from 0.448 to 0.909, respectively. These SSR markers will be available to identify genets and evaluate genetic diversity of V. album ssp. oxysepalum.
我们使用双重抑制聚合酶链反应技术和改良方法，从日本北海道种群的基因组 DNA 中开发了 11 个多态性微卫星[简单序列重复 (SSR)] 位点。这些标记物在 48 个样本中检测到 47 种基因型，每个位点有 4 到 17 个等位基因。观察到的和预期的杂合度分别在 0.042 到 0.938 和 0.448 到 0.909 之间。这些 SSR 标记物将可用于鉴定 V. album ssp. oxysepalum 的遗传体并评估其遗传多样性。
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