大姚山荷叶（苦苣苔科）微卫星标记，一种极危的多年生草本植物。
Microsatellite markers for Dayaoshania cotinifolia (Gesneriaceae), a critically endangered perennial herb.
机构信息
College of Life Sciences, Henan Agricultural University, Zhengzhou 450002, China.
出版信息
Am J Bot. 2011 Sep;98(9):e256-8. doi: 10.3732/ajb.1100170. Epub 2011 Aug 29.
PREMISE OF THE STUDY
Microsatellite primers were developed for the critically endangered species Dayaoshania cotinifolia (Gesneriaceae) to evaluate population genetic diversity and detect population history. •
METHODS AND RESULTS
In our study, 15 primer sets were developed using an enriched genomic library. These are the first microsatellite loci developed for this genus. Genetic diversity was measured using 40 individuals. The number of alleles per locus ranged from one to six, and the incidence of observed heterozygosities was 0.365 and 0.410 in two populations. •
CONCLUSIONS
The described markers will be useful in future population genetics studies of this critically endangered species.
研究前提
为濒危物种大冶猕猴桃 (苦苣苔科) 开发微卫星引物，以评估种群遗传多样性并检测种群历史。
方法与结果
在本研究中，使用富集基因组文库开发了 15 对引物。这是该属的第一批微卫星基因座。使用 40 个个体测量遗传多样性。每个基因座的等位基因数范围从一个到六个，两个群体的观察杂合度分别为 0.365 和 0.410。
结论
描述的标记将对该濒危物种的未来种群遗传学研究有用。
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