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18 个来自高山湖泊种群的虹鳟克隆的四核苷酸微卫星 DNA 标记。

Eighteen new tetranucleotide microsatellite DNA markers for Coregonus lavaretus cloned from an alpine lake population.

机构信息

Institute of Zoology, Universitätsplatz 2, A-8010 Graz, Austria.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):1055-8. doi: 10.1111/j.1755-0998.2008.02153.x. Epub 2008 Jun 28.

Abstract

We developed 18 polymorphic microsatellite markers for Coregonus lavaretus from genomic libraries enriched for (GACA)(n) and (GATA)(n) repeat sequences. Emphasis was placed on developing highly polymorphic, perfect repeats. These loci were screened in 69 individuals from two alpine populations in Austria. Allelic variation was high with nine to 37 alleles per locus and expected heterozygosities ranging from 0.37 to 0.95. The high level of polymorphism revealed by these loci will be relevant for population studies in context to the evolutionary history of this species.

摘要

我们从富含 (GACA)(n) 和 (GATA)(n) 重复序列的基因组文库中开发了 18 个用于湖红点鲑的多态微卫星标记。重点是开发高度多态、完美的重复序列。这些位点在奥地利两个高山种群的 69 个个体中进行了筛选。每个位点有 9 到 37 个等位基因,预期杂合度从 0.37 到 0.95 不等,等位基因变异程度很高。这些位点揭示的高水平多态性将与该物种的进化历史有关,对种群研究具有重要意义。

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