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用于蟾科巨头螈(Dicamptodon copei)和太平洋巨型蝾螈(Dicamptodon tenebrosus)的多态四核苷酸微卫星。

Polymorphic tetranucleotide microsatellites for Cope's giant salamander (Dicamptodon copei) and Pacific giant salamander (Dicamptodon tenebrosus).

机构信息

School of Biological Sciences, Washington State University, Pullman, WA 99164-4236, USA.

出版信息

Mol Ecol Resour. 2008 Sep;8(5):1071-3. doi: 10.1111/j.1755-0998.2008.02158.x. Epub 2008 Jun 28.

Abstract

We present primers and amplification conditions for 15 microsatellite loci developed for the Cope's giant salamander (Dicamptodon copei), 14 of which are tetranucleotide repeats. Cross-species amplification revealed 10 of these loci to also be polymorphic in the Pacific giant salamander (Dicamptodon tenebrosus). Several loci produced nonoverlapping allelic ranges between the two species and may be useful in species identification. These polymorphic microsatellite loci are potentially useful for future studies of population genetics in dicamptodontid salamanders.

摘要

我们提出了为科氏巨螈(Dicamptodon copei)开发的 15 个微卫星基因座的引物和扩增条件,其中 14 个是四核苷酸重复。跨物种扩增显示,这 10 个基因座在太平洋巨螈(Dicamptodon tenebrosus)中也是多态的。一些基因座在两个物种之间产生了不重叠的等位基因范围,可能对物种鉴定有用。这些多态性微卫星基因座可能对未来研究双齿螈的群体遗传学有用。

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