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雌雄异株的石竹属植物(Silene latifolia 和 S. dioica)的高效分子性别鉴定以及 F1 杂种的亲权分析。

Efficient molecular sexing in dioecious Silene latifolia and S. dioica and paternity analysis in F(1) hybrids.

机构信息

Laboratory of Plant Developmental Genetics, Institute of Biophysics, Academy of Sciences of the Czech Republic, Kralovopolska 135, CZ-612 65 Brno, Czech Republic, Plant Ecological Genetics, Institute of Integrative Biology, ETH Zurich, Universitaetstr. 16, 8092 Zurich, Switzerland.

出版信息

Mol Ecol Resour. 2008 Nov;8(6):1274-6. doi: 10.1111/j.1755-0998.2008.02344.x.

Abstract

Two polymerase chain reaction-based assays have been developed that work in combination with an efficient DNA extraction protocol to rapidly and reliably determine sex in the dioecious plant species Silene latifolia and S. dioica. In addition, one of the assays allows assessing paternity in the F(1) generation of intra- and interspecific matings involving the two species.

摘要

已经开发出两种基于聚合酶链反应的检测方法,与一种高效的 DNA 提取方案相结合,可以快速可靠地确定雌雄异株植物石竹属植物和 S. dioica 的性别。此外,其中一种检测方法还可以评估涉及两种植物的种内和种间交配的 F(1)代中的亲权。

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