Ecological Genetics Research Unit, Department of Biological and Environmental Sciences, PO Box 65, FI-00014, University of Helsinki, Finland.
Mol Ecol Resour. 2008 Nov;8(6):1469-74. doi: 10.1111/j.1755-0998.2008.02223.x. Epub 2008 Aug 20.
We describe primers and polymerase chain reaction conditions to amplify 100 microsatellite loci from the Siberian jay (Perisoreus infaustus). The primers were tested on two geographically separated Finnish populations. The developed primer pairs yielded an average of 4.72 alleles per locus (range one to 17) and an average observed heterozygosity of 0.55 (range 0.04 to 1).
我们描述了从松鸦(Perisoreus infaustus)中扩增 100 个微卫星位点的引物和聚合酶链反应条件。这些引物在两个地理位置分开的芬兰人群中进行了测试。开发的引物对每个位点平均产生 4.72 个等位基因(范围为 1 到 17),平均观测杂合度为 0.55(范围为 0.04 到 1)。