用于东方铁杉（Tsuga canadensis）的微卫星标记。
Microsatellite markers for eastern hemlock (Tsuga canadensis).
机构信息
D. B. Warnell School of Forestry and Natural Resources, The University of Georgia, Athens, GA 30602, USA, Southern Research Station, Southern Institute of Forest Genetics, USDA Forest Service, Saucier, MS 39574, USA.
出版信息
Mol Ecol Resour. 2008 Nov;8(6):1354-6. doi: 10.1111/j.1755-0998.2008.02274.x. Epub 2008 Sep 3.
We describe polymerase chain reaction primer pairs and reaction conditions for amplification of 15 microsatellite loci from eastern hemlock (Tsuga canadensis). The primers were tested on 23 individuals from a natural population in southwestern North Carolina, USA. These primers yielded an average of 5.9 alleles per locus (range of 2-14), an average observed heterozygosity of 0.45 (range 0.14-0.73), and an average polymorphic information content of 0.54 (range 0.28-0.86). In addition, eight of the primer pairs were found to amplify microsatellite loci in one or more additional species of Tsuga.
我们描述了从东方铁杉（Tsuga canadensis）中扩增 15 个微卫星位点的聚合酶链反应引物对和反应条件。我们在美国北卡罗来纳州西南部的一个自然种群中对 23 个个体进行了测试。这些引物在每个位点平均产生 5.9 个等位基因（范围为 2-14），平均观察杂合度为 0.45（范围为 0.14-0.73），平均多态信息含量为 0.54（范围为 0.28-0.86）。此外，有 8 对引物被发现可以扩增 Tsuga 一个或多个其他物种中的微卫星位点。
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