Chang Dong Woo, Kim Bora, Shin Jae Hoon, Yun Young Min, Je Jung Ho, Hwu Yeu Kuang, Yoon Jung Hee, Seong Je Kyung
Department of Radiology, College of Veterinary Medicine, Chungbuk National University, Cheongju 361-763, Korea.
J Vet Sci. 2011 Jun;12(2):107-13. doi: 10.4142/jvs.2011.12.2.107.
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under anesthesia at embryonic day (E)12, E14, E15, and E18. At the same time, conventional radiography and mammography were used to compare with X-ray synchrotron. After synchrotron radiation, each mouse was sacrificed and stained with Alizarin red S and Alcian blue to observe bony structures. Synchrotron radiation enabled us to view the mouse fetal skeleton beginning at gestation. Synchrotron radiation systems facilitate real time observations of the fetal skeleton with greater accuracy and magnification compared to mammography and conventional radiography. Our results show that X-ray synchrotron systems can be used to observe the fine structures of internal organs at high magnification.
X射线同步加速器与传统辐射源有很大不同。这项技术可能会扩展传统放射学的能力，并以新颖的方式应用于特殊情况。为了评估X射线同步加速器辐射系统用于实时观察的效用，使用高分辨率X射线同步加速器监测小鼠胎儿骨骼发育。采用非单色X射线同步加速器（白色光束，5C1光束线）观察处于胚胎期第12天、第14天、第15天和第18天的麻醉小鼠的骨骼。同时，使用传统放射摄影和乳腺摄影与X射线同步加速器进行比较。同步加速器辐射后，处死每只小鼠并用茜素红S和阿尔新蓝染色以观察骨骼结构。同步加速器辐射使我们能够从妊娠开始就观察到小鼠胎儿骨骼。与乳腺摄影和传统放射摄影相比，同步加速器辐射系统有助于更准确、更大倍率地实时观察胎儿骨骼。我们的结果表明，X射线同步加速器系统可用于高倍率观察内部器官的精细结构。
