Farley Frances A, Hall Jan, Goldstein Steven A
Department of Orthopedic Surgery, Orthopedic Research Lab, University of Michigan, Ann Arbor, MI 48109, USA.
J Pediatr Orthop. 2006 May-Jun;26(3):341-6. doi: 10.1097/01.bpo.0000203011.58529.d8.
Pregnant mice were exposed to 600 ppm of carbon monoxide on gestation day 9. The carbon monoxide exposure produced 38 spinal anomalies in 17 offspring with congenital scoliosis. Microcomputer tomography improved the identification of spinal anomalies and detected more affected animals, as compared when magnified radiographs were used. The curves were measured and ranged from mild to moderate at an age that corresponds to childhood and, on average, remained at the range of mild to moderate through near-maturity. The group of mice with congenital anomalies near maturity contained severe curves. The bone mineral density (BMD) determined by microcomputer tomography in the mice with congenital scoliosis was the same as that in the controls. The cervical spine BMD was higher than the thoracic BMD, which was higher than the lumbar region in the controls and affected animals. The BMD was highest in the apex of the congenital curves in the animals that were near maturity. This relationship was not seen in the younger mice with congenital curves. Histology revealed growth rates in the hemivertebrae, which, over the last half of the growth period measured, had one-third less growth than the adjacent vertebrae. The hemivertebrae were wedged, but the adjacent disks were not.
妊娠第9天的怀孕小鼠暴露于600 ppm的一氧化碳中。一氧化碳暴露导致17只患有先天性脊柱侧凸的后代出现38处脊柱异常。与使用放大X线照片相比，微型计算机断层扫描改善了脊柱异常的识别，并检测出更多受影响的动物。这些曲线在相当于儿童期的年龄进行测量，范围从轻度到中度，并且平均而言，在接近成熟时仍保持在轻度到中度的范围内。接近成熟时患有先天性异常的小鼠组包含严重的曲线。通过微型计算机断层扫描测定的先天性脊柱侧凸小鼠的骨矿物质密度（BMD）与对照组相同。对照组和受影响动物的颈椎BMD高于胸椎BMD，而胸椎BMD又高于腰椎区域。在接近成熟的动物中，先天性曲线顶点处的BMD最高。在患有先天性曲线的较年轻小鼠中未观察到这种关系。组织学显示半椎体的生长速率，在所测量的生长后期，其生长比相邻椎体少三分之一。半椎体呈楔形，但相邻椎间盘未出现这种情况。
