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牛卵母细胞成熟和早期胚胎发育过程中Ca²⁺水平的动态分析

Dynamic analysis of Ca²+ level during bovine oocytes maturation and early embryonic development.

作者信息

Liang Su Li, Zhao Qian Jun, Li Xiang Chen, Jin Ya Ping, Wang Yi Peng, Su Xiao Hua, Guan Wei Jun, Ma Yue Hui

机构信息

College of Veterinary Medicine, Northwest Agriculture and Forestry University, Yangling 712100, China.

出版信息

J Vet Sci. 2011 Jun;12(2):133-42. doi: 10.4142/jvs.2011.12.2.133.

Abstract

Mammalian oocyte maturation and early embryo development processes are Ca(2+)-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca(2+) and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca(2+) was distributed in the cortical ooplasm and throughout the oocytes from the MI to MII stage. In IVF embryos, Ca(2+) was distributed in the cortical ooplasm before the formation of the pronucleus. In 4-8 cell embryos and morulas, Ca(2+) was present throughout the blastomere. In PA embryos, Ca(2+) was distributed throughout the blastomere at 48 h, similar to in the 4-cell and 8-cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca(2+) in the SCNT embryos. However, Ca(2+) was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca(2+) showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca(2+) location and distribution changes during oocyte maturation and early embryo development processes should facilitate a better understanding of the mechanisms involved in oocyte maturation, reconstructed embryo activation and development, ultimately improving the reconstructed embryo development rate.

摘要

哺乳动物卵母细胞成熟和早期胚胎发育过程是依赖钙离子的。在本研究中,我们使用共聚焦显微镜来研究钙离子在牛卵母细胞成熟、体外受精(IVF)、孤雌激活(PA)和体细胞核移植(SCNT)胚胎发育过程中的分布模式及其动态变化。在生发泡(GV)期和GV破裂阶段,钙离子分布于皮质卵质中,并且从减数第一次分裂中期(MI)到减数第二次分裂中期(MII)阶段遍布整个卵母细胞。在IVF胚胎中,在原核形成之前钙离子分布于皮质卵质中。在4-8细胞胚胎和桑椹胚中,钙离子存在于整个卵裂球中。在PA胚胎中,48小时时钙离子遍布整个卵裂球,类似于4细胞期、8细胞期和桑椹胚。激活后6小时,SCNT胚胎中几乎没有钙离子分布。然而,10小时时钙离子分布于供体细胞核中,并且在2-8细胞胚胎中遍布整个卵裂球。在本研究中,钙离子在IVF和SCNT组中呈现出有规律的显著波动,但PA组没有。系统研究卵母细胞成熟和早期胚胎发育过程中钙离子的定位和分布变化,应有助于更好地理解卵母细胞成熟、重构胚胎激活和发育所涉及的机制,最终提高重构胚胎的发育率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/00b8/3104167/8e96344c969b/jvs-12-133-g001.jpg

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