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人支气管组织中环核苷酸磷酸二酯酶的部分纯化及特性研究

Partial purification and characterization of cyclic nucleotide phosphodiesterases from human bronchial tissue.

作者信息

Bergstrand H, Lundquist B

出版信息

Mol Cell Biochem. 1978 Oct 13;21(1):9-15. doi: 10.1007/BF00230191.

DOI:10.1007/BF00230191
PMID:215899
Abstract

DEAE-Sephadex chromatography reveals the presence in extracts of human bronchial tissue of at least three separate cyclic nucleotide phosphodiesterases: a cyclic GMP-specific high affinity enzyme, a non-specific low affinity enzyme, and a high affinity cyclic AMP-specific enzyme. The activity of each fraction was partially characterized with respect to kinetic parameters, thermal stability, and the influence of a number of inhibitors. Each activity was found to resemble the activity of the previously characterized corresponding enzyme from whole lung tissue extracts. A high affinity non-specific phospho-diesterase previously isolated from lung tissue is missing in extracts of bronchial tissue.

摘要

二乙氨基乙基葡聚糖柱层析显示，人支气管组织提取物中至少存在三种不同的环核苷酸磷酸二酯酶：一种对环鸟苷酸具有特异性的高亲和力酶、一种非特异性低亲和力酶和一种高亲和力的环腺苷酸特异性酶。对每个组分的活性就动力学参数、热稳定性以及多种抑制剂的影响进行了部分表征。发现每种活性都类似于先前从全肺组织提取物中鉴定出的相应酶的活性。先前从肺组织中分离出的一种高亲和力非特异性磷酸二酯酶在支气管组织提取物中不存在。

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引用本文的文献

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Effects of antiallergic agents, compound 48/80, and some reference inhibitors on the activity of partially purified human lung tissue adenosine cyclic 3',5'-monophosphate and guanosine cyclic 3',5'-monophosphate phosphodiesterases.抗过敏剂、化合物48/80及一些参考抑制剂对部分纯化的人肺组织腺苷环3',5'-单磷酸和鸟苷环3',5'-单磷酸磷酸二酯酶活性的影响。
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Inhibitory and activators of cyclic nucleotide phosphodiesterase.环核苷酸磷酸二酯酶的抑制剂和激活剂。
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