Stclair D, Wan X, Kuroda M, Vichitbandha S, Tsuchida E, Urano M
UNIV KENTUCKY,MED CTR,DEPT RADIAT MED,LEXINGTON,KY 40536.
Oncol Rep. 1997 Jul-Aug;4(4):753-7. doi: 10.3892/or.4.4.753.
We have examined the tumorigenicity and the level of extracellular matrix proteins in fibrosarcoma cells expressing low (SOD-L) and high (SOD-H) MnSOD activities as well as the fibrosarcoma cells transfected with the selectable marker alone (NEO). When the cells derived from each tumor cell line were injected into syngeneic mice., the number of tumor cells required to make a tumor in one-half of the mice (TD50) was markedly increased in MnSOD-transfected cells. The decrease in the tumorigenicity of the MnSOD-transfected cells was associated with an increase in the fibronectin level. These results support the hypothesis that MnSOD is a new type of tumor-suppressor gene.
我们检测了表达低（SOD-L）和高（SOD-H）锰超氧化物歧化酶活性的纤维肉瘤细胞以及仅转染了选择标记（NEO）的纤维肉瘤细胞的致瘤性和细胞外基质蛋白水平。当将来自每个肿瘤细胞系的细胞注射到同基因小鼠体内时，锰超氧化物歧化酶转染细胞中使一半小鼠形成肿瘤所需的肿瘤细胞数量（TD50）显著增加。锰超氧化物歧化酶转染细胞致瘤性的降低与纤连蛋白水平的增加有关。这些结果支持了锰超氧化物歧化酶是一种新型肿瘤抑制基因的假说。
