Yu J, Reed E, Mu C, Bostickbruton F
NCI,MED OVARIAN CANC SECT,MED BRANCH,DEV THERAPEUT DEPT,DIV CLIN SCI,BETHESDA,MD 20892.
Oncol Rep. 1997 Sep-Oct;4(5):905-7. doi: 10.3892/or.4.5.905.
New methodology is important to the advancement of biomedical science. We recently described a polymorphism within exon IV of the ERCC1 gene, which is associated with an approximate 50% decrease in codon usage in some biological systems. In this report we show that this polymorphism can be readily demonstrated by standard manual DNA sequencing, and by a recently developed methodology which relies on spectrophotometric principles. The major advantage of this new methodology is that several-fold more samples can be assessed per unit time, at reduced cost.
新方法对生物医学科学的进步至关重要。我们最近描述了ERCC1基因第四外显子内的一种多态性，在某些生物系统中，这种多态性与密码子使用频率下降约50%相关。在本报告中，我们表明这种多态性可以通过标准的手动DNA测序以及一种基于分光光度原理的最新开发方法轻松检测到。这种新方法的主要优点是，每单位时间可以评估的样本数量增加了几倍，成本却降低了。
