Chen Haifeng
Virovek, Inc., San Francisco, CA, USA.
Methods Mol Biol. 2011;737:235-46. doi: 10.1007/978-1-61779-095-9_10.
Adeno-associated virus (AAV) is one of the most promising vectors for gene therapy. There are several ways of producing AAV vectors but large-scale production of this vector remains a major challenge. Virovek developed a novel method of expressing the AAV Rep and Cap genes in insect cells mediated by intron-splicing mechanism and producing AAV vectors with these Rep and Cap sequences containing the artificial intron. The recombinant baculoviruses harboring these artificial intron-containing Rep and Cap sequences are very stable and the AAV vectors produced in insect cells with these recombinant baculoviruses are very infectious.
腺相关病毒（AAV）是基因治疗中最有前景的载体之一。生产AAV载体有多种方法，但这种载体的大规模生产仍然是一个重大挑战。Virovek开发了一种新方法，通过内含子剪接机制在昆虫细胞中表达AAV Rep和Cap基因，并利用含有这些Rep和Cap序列及人工内含子的序列生产AAV载体。携带这些含人工内含子的Rep和Cap序列的重组杆状病毒非常稳定，利用这些重组杆状病毒在昆虫细胞中生产的AAV载体具有很强的感染性。
